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硫氧化还原蛋白(Trx)多克隆抗体

Polyclonal Antibody to Thioredoxin (Trx)

TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein

  • 硫氧化还原蛋白(Trx)多克隆抗体 产品包装(模拟)
  • 硫氧化还原蛋白(Trx)多克隆抗体 产品包装(模拟)
  • 硫氧化还原蛋白(Trx)多克隆抗体 FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human Trx Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • PAA702Hu01.jpg Figure. Western Blot; Sample: Recombinant Trx, Human.
  • 硫氧化还原蛋白(Trx)多克隆抗体 Figure. Western Blot; Sample: Lane1: Human hepG2 Cells; Lane2: Human MCF7 Cells; Lane3: Human Hela Cells.
  • 硫氧化还原蛋白(Trx)多克隆抗体 HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human Trx Polyclonal Antibody (Catalog # PAA702Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • 硫氧化还原蛋白(Trx)多克隆抗体 Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human Trx Polyclonal Antibody (Catalog PAA702Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对Trx的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Trx。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA702Hu01 硫氧化还原蛋白(Trx)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA702Hu01 硫氧化还原蛋白(Trx)多克隆抗体 WB; IHC; ICC; IP; FCM
FAA702Hu01 抗硫氧化还原蛋白(Trx)多克隆抗体 Flow cytometry.
LAA702Hu81 硫氧化还原蛋白(Trx)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA702Hu71 硫氧化还原蛋白(Trx)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA702Hu25 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu27 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu26 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu29 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu28 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu22 硫氧化还原蛋白(Trx)单克隆抗体 WB
MAA702Hu21 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu23 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
MAA702Hu24 硫氧化还原蛋白(Trx)单克隆抗体 WB; IHC; ICC; IP.
FAA702Hu08 抗硫氧化还原蛋白(Trx)单克隆抗体 Flow cytometry.
FAA702Hu06 抗硫氧化还原蛋白(Trx)单克隆抗体 Flow cytometry.
FAA702Hu04 抗硫氧化还原蛋白(Trx)单克隆抗体 Flow cytometry.
FAA702Hu02 抗硫氧化还原蛋白(Trx)单克隆抗体 Flow cytometry.
SEA702Hu 硫氧化还原蛋白(Trx)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA702Hu 硫氧化还原蛋白(Trx)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA702Hu 硫氧化还原蛋白(Trx)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Human Reproduction The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis [OxfordJournals: source]
Exp Ther Med. Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus [Pubmed:Pmc4061188]
Reproduction Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627]
Free Radical Research Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress [Pubmed:25564095]
Antioxidants Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. [Pubmed:26797643]
FASEB Journal Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the … [Pubmed: 30351992]
World Journal of Gastrointestinal Oncology Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer [Doi: 10.4251/wjgo.v11.i2.117]
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479]
European Journal of Pharmacology Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitus [Pubmed: 31351037]
Journal of Clinical Medicine Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
HISTOCHEMISTRY AND CELL BIOLOGY Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response [Pubmed: 33161477]
Journal of Cosmetic Dermatology Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583]
Heliyon Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats [34159266]
Hum Exp Toxicol Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936]
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