干扰素调节因子3(IRF3)多克隆抗体
Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
- 编号PAB589Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPB589Hu01-干扰素调节因子3(IRF3)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
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Figure: FITC staining on IHC-P;
Sample: Hela cells. -
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11) -
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: Rat Kidney lysate Primary Ab: 0.6µg/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human IRF3 Polyclonal Antibody (Catalog PAB589Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对IRF3的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别IRF3。
用法
Western blotting: 0.01-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB589Hu01 | 干扰素调节因子3(IRF3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB589Hu01 | 干扰素调节因子3(IRF3)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| MAB589Hu22 | 干扰素调节因子3(IRF3)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB589Hu | 干扰素调节因子3(IRF3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB589Hu | 干扰素调节因子3(IRF3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLOS Pathogens | RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production [pubmed:29186193] |
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