角蛋白8(CK8)多克隆抗体
Polyclonal Antibody to Cytokeratin 8 (CK8)
K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8
- 编号PAC025Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPC025Hu01-角蛋白8(CK8)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种-
- 应用WB,IHC,ICC/IF; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CK8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant CK8, Human.
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Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: KRT8 knockout Hela cell lysate;
Predicted MW: 54,57kDa
Observed MW: 57kDa
Primary Ab: 2µg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate
Primary Ab: 0.05µg/ml Rabbit Anti-Human CK8 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK8 Polyclonal Antibody (Catalog PAC025Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CK8的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CK8。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC025Hu01 | 角蛋白8(CK8)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC025Hu02 | 角蛋白8(CK8)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC025Hu01 | 角蛋白8(CK8)多克隆抗体 | WB,IHC,ICC/IF; FCM |
| LAC025Hu81 | 角蛋白8(CK8)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAC025Hu71 | 角蛋白8(CK8)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC025Hu21 | 角蛋白8(CK8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC025Hu23 | 角蛋白8(CK8)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC025Hu22 | 角蛋白8(CK8)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC025Hu | 角蛋白8(CK8)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC025Hu | 角蛋白8(CK8)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Molecular Medicine Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes [Pubmed:29749501] |
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