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角蛋白8(CK8)多克隆抗体

Polyclonal Antibody to Cytokeratin 8 (CK8)

K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8

  • 角蛋白8(CK8)多克隆抗体 产品包装(模拟)
  • 角蛋白8(CK8)多克隆抗体 产品包装(模拟)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CK8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Tonsil Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Thyroid cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAC025Hu01.jpg Figure. Western Blot; Sample: Recombinant CK8, Human.
  • 角蛋白8(CK8)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: KRT8 knockout Hela cell lysate;
    Predicted MW: 54,57kDa
    Observed MW: 57kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 Western Blot; Sample: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CK8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 角蛋白8(CK8)多克隆抗体 Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK8 Polyclonal Antibody (Catalog PAC025Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CK8的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CK8。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC025Hu01 角蛋白8(CK8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC025Hu02 角蛋白8(CK8)多克隆抗体 WB; IHC; ICC; IP.
PAC025Hu01 角蛋白8(CK8)多克隆抗体 WB,IHC,ICC/IF; FCM
LAC025Hu81 角蛋白8(CK8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC025Hu71 角蛋白8(CK8)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC025Hu21 角蛋白8(CK8)单克隆抗体 WB; IHC; ICC; IP.
MAC025Hu23 角蛋白8(CK8)单克隆抗体 WB; IHC; ICC; IP.
MAC025Hu22 角蛋白8(CK8)单克隆抗体 WB; IHC; ICC; IP.
SEC025Hu 角蛋白8(CK8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC025Hu 角蛋白8(CK8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Molecular Medicine Reports Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes [Pubmed:29749501]
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