Smad同源物3(SMAD3)多克隆抗体
Polyclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- 编号PAC123Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPC123Hu01-Smad同源物3(SMAD3)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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IHC图
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Figure. Western Blot; Sample: Recombinant Smad3, Human.
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Western Blot; Sample: Rat Pancreas lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: Smad3 knockout PC3 cell lysate;
Predicted MW: 26,36,43,48kd
Observed MW: 43kd
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human PC3 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对SMAD3的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别SMAD3。
用法
Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC123Hu01 | Smad同源物3(SMAD3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Smad同源物3(SMAD3)多克隆抗体 | WB; IHC; ICC; IP. |
| FAC123Hu01 | 抗Smad同源物3(SMAD3)多克隆抗体 | Flow cytometry. |
| MAC123Hu21 | Smad同源物3(SMAD3)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC123Hu22 | Smad同源物3(SMAD3)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC123Hu23 | Smad同源物3(SMAD3)单克隆抗体 | WB; IHC; ICC; IP. |
参考文献
| 杂志 | 参考文献 |
| У¿¯ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model [] |
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