乙酰辅酶A乙酰转移酶1(ACAT1)多克隆抗体
Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)
THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase
- 编号PAD664Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPD664Hu01-乙酰辅酶A乙酰转移酶1(ACAT1)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.71mg/ml
- 且适物种Sus scrofa; Porcine (Pig，猪)
- 应用IHC; IF; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: ACAT1 knockout A549 cell lysate;
Predicted MW: 17,45kd
Observed MW: 44kd
Primary Ab: 1µg/ml Rabbit Anti-Human ACAT1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACAT1 Polyclonal Antibody (Catalog PAD664Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对ACAT1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别ACAT1。
用法
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD664Hu01 | 乙酰辅酶A乙酰转移酶1(ACAT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD664Hu01 | 乙酰辅酶A乙酰转移酶1(ACAT1)多克隆抗体 | IHC; IF; FCM |
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