细胞外基质金属蛋白酶诱导因子(CD147)多克隆抗体
Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
- 编号PAB540Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPB540Mu01-细胞外基质金属蛋白酶诱导因子(CD147)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度500µg/mL
- 且适物种Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Heart Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Intestine Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant EMMPRIN, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse CD147 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Heart lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Thymocytes cell were stained with 20µg/ml rabbit Anti-mouse CD147 Polyclonal Antibody (Catalog PAB540Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CD147的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD147。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB540Mu61 | 细胞外基质金属蛋白酶诱导因子(CD147)活性蛋白 | Cell culture; Activity Assays. |
| APB540Mu02 | 细胞外基质金属蛋白酶诱导因子(CD147)活性蛋白 | Cell culture; Activity Assays. |
| RPB540Mu01 | 细胞外基质金属蛋白酶诱导因子(CD147)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB540Mu02 | 细胞外基质金属蛋白酶诱导因子(CD147)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB540Mu01 | 细胞外基质金属蛋白酶诱导因子(CD147)活性蛋白 | Cell culture; Activity Assays. |
| EPB540Mu61 | 细胞外基质金属蛋白酶诱导因子(CD147)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB540Mu01 | 细胞外基质金属蛋白酶诱导因子(CD147)多克隆抗体 | WB; IHC; ICC; IP; FCM |
参考文献
| 杂志 | 参考文献 |
| DISEASE MARKERS | The Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung Cancer [Pubmed: 32377273] |
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