己糖激酶2(HK2)多克隆抗体
Polyclonal Antibody to Hexokinase 2 (HK2)
HK II; Muscle form hexokinase
- 编号PAD352Hu02
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPD352Hu02-己糖激酶2(HK2)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状液体
- 浓度500µg/mL
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB; IHC; ICC; IP; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 10µg/ml Rabbit Anti-Human HK2 Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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Figure: FITC staining on IHC-P;
Sample: MCF7 cells.
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WB图
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: HK2 knockout MCF7 cell lysate;
Predicted MW: 102kd
Observed MW: 102kd
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human HK2 Polyclonal Antibody (Catalog PAD352Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对HK2的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别HK2。
用法
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD352Hu01 | 己糖激酶2(HK2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD352Hu02 | 己糖激酶2(HK2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD352Hu01 | 己糖激酶2(HK2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAD352Hu02 | 己糖激酶2(HK2)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| MAD352Hu21 | 己糖激酶2(HK2)单克隆抗体 | WB; IHC; ICC; IP. |
| SED352Hu | 己糖激酶2(HK2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD352Hu | 己糖激酶2(HK2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Anti-Cancer Drugs | 3-Bromopyruvate enhances TRAIL-induced apoptosis in human nasopharyngeal carcinoma cells through CHOP-dependent upregulation of TRAIL-R2. [10.1097/CAD.0000000000000502] |
| Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) [] | |
| J Cell Mol Med | Huaier shows anti©\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF©\1¦Á pathway [33377619] |
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