蛋白二硫化物异构酶A4(PDIA4)多克隆抗体
Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)
PDI-A4; ERP70; ERP72; Protein Disulfide Isomerase-Associated 4; Endoplasmic reticulum resident protein 70
- 编号PAD774Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPD774Hu01-蛋白二硫化物异构酶A4(PDIA4)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度500µg/mL
- 且适物种Sus scrofa; Porcine (Pig，猪)
- 应用WB,IHC
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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Figure. FITC staining on IHC-P; Samples: Human Hela Cells.
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DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Pancreas Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant PDIA4, Human.
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Porcine Liver lysate; Lane3: Porcine Cerebrum lysate; Lane4: A431 cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: PDIA4 knockout Hela cell lysate;
Predicted MW: 73kd
Observed MW: 73kd
Primary Ab: 3µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: NIH/3T3 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对PDIA4的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PDIA4。
用法
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD774Hu01 | 蛋白二硫化物异构酶A4(PDIA4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD774Hu01 | 蛋白二硫化物异构酶A4(PDIA4)多克隆抗体 | WB,IHC |
| MAD774Hu21 | 蛋白二硫化物异构酶A4(PDIA4)单克隆抗体 | WB; IHC; ICC; IP. |
| SED774Hu | 蛋白二硫化物异构酶A4(PDIA4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD774Hu | 蛋白二硫化物异构酶A4(PDIA4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Indonesian Journal of Clinical Pathology and Medical Laboratory | Keluarga Disulfit Protein Isomerase Anggota 4(PDIA4) di Kanker Payudara dengan metastasis [Unair.ac: Source] |
| PLoS One | A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4 [pubmed:28650993] |
| Acta Diabetol | Circulating protein disulfide isomerase family member 4 is associated with type 2 diabetes mellitus, insulin sensitivity, and obesity [Pubmed:35460376] |
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