27kDa热休克蛋白(Hsp27)单克隆抗体
Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- 编号MAA693Hu21
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)c2
- Ig亚型 IgG1 Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原 RPA693Hu01-27kDa热休克蛋白(Hsp27)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/mL
- 且适物种Sus scrofa; Porcine (Pig，猪)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Sample: Human Glioma Tissue; Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Skin cancer Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Brain Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Porcine Heart lysate Primary Ab: 2µg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2µg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog MAA693Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对Hsp27的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Hsp27。
用法
Western blottin:g: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA693Hu61 | 27kDa热休克蛋白(Hsp27)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA693Hu01 | 27kDa热休克蛋白(Hsp27)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA693Hu61 | 27kDa热休克蛋白(Hsp27)活性蛋白 | Cell culture; Activity Assays. |
| PAA693Hu01 | 27kDa热休克蛋白(Hsp27)多克隆抗体 | WB,IHC,ICC/IF |
| FAA693Hu01 | 抗27kDa热休克蛋白(Hsp27)多克隆抗体 | FCM |
| LAA693Hu81 | 27kDa热休克蛋白(Hsp27)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA693Hu71 | 27kDa热休克蛋白(Hsp27)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| FAA693Hu81 | 抗27kDa热休克蛋白(Hsp27)多克隆抗体(异硫氰酸荧光素标记) | Flow cytometry. |
| MAA693Hu22 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA693Hu21 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA693Hu23 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC |
| MAA693Hu24 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA693Hu25 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA693Hu27 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA693Hu29 | 27kDa热休克蛋白(Hsp27)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA693Hu06 | 抗27kDa热休克蛋白(Hsp27)单克隆抗体 | Flow cytometry. |
| FAA693Hu08 | 抗27kDa热休克蛋白(Hsp27)单克隆抗体 | Flow cytometry. |
| FAA693Hu04 | 抗27kDa热休克蛋白(Hsp27)单克隆抗体 | Flow cytometry. |
| FAA693Hu02 | 抗27kDa热休克蛋白(Hsp27)单克隆抗体 | Flow cytometry. |
| LAA693Hu74 | 27kDa热休克蛋白(Hsp27)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA693Hu | 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA693Hu | 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA693Hu | 27kDa热休克蛋白(Hsp27)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA693Hu01 | 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
| Nature Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953] |
| Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
| Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients [] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771] |
| Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation [] |
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