泛素羧基端酯酶L1(UCHL1)多克隆抗体
Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 编号PAG945Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPG945Mu01-泛素羧基端酯酶L1(UCHL1)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.44mg/ml
- 且适物种Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Colon Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Pancreas Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant UCHL1, Mouse.
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Western Blot; Sample: Mouse Testis lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Pancreas lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse UCHL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse UCHL1 Polyclonal Antibody (Catalog PAG945Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对UCHL1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别UCHL1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPG945Mu02 | 泛素羧基端酯酶L1(UCHL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPG945Mu01 | 泛素羧基端酯酶L1(UCHL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG945Mu01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAG945Mu02 | 泛素羧基端酯酶L1(UCHL1)多克隆抗体 | WB; IHC; ICC; IP. |
| SEG945Mu | 泛素羧基端酯酶L1(UCHL1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG945Mu | 泛素羧基端酯酶L1(UCHL1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSG945Mu01 | 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
| Intensive Care Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |
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