细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组抗体
Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)
CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4
- 编号RAB230Hu71
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源重组单抗制备
- 宿主CHO
- 效价(克隆号)-
- Ig亚型 IgG
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物生物素
- 免疫原 -
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度1mg/mL
- 且适物种-
- 应用WB; IF; ICC; IHC; IP; FCM.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 1080 ¥ 2520 ¥ 3600 ¥ 9000 ¥ 36000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Briefly, biotin labelled CTLA4 Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to rh CTLA4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of rh CTLA4 and CTLA4 Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.06607 ug/ml.
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CTLA4的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CTLA4。
用法
免疫印迹：0.2-2µg/mL;1:500-5000
免疫组织化学：5-20µg/mL;1:50-200
免疫细胞化学：5-20µg/mL;1:50-200
最佳稀释倍数最终由用户决定。
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB230Hu01 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB230Hu03 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB230Hu02 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB230Hu04 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB230Hu02 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB230Hu01 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体 | WB; IHC; ICC; IP. |
| FAB230Hu01 | 抗细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体 | Flow cytometry. |
| FAB230Hu41 | 抗细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体(藻红素标记) | Flow cytometry. |
| FAB230Hu51 | 抗细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体(别藻蓝蛋白标记) | Flow cytometry. |
| FAB230Hu81 | 抗细胞毒性T-淋巴细胞关联抗原4(CTLA4)多克隆抗体(异硫氰酸荧光素标记) | Flow cytometry. |
| MAB230Hu21 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB. |
| MAB230Hu23 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| RAB230Hu71 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAB230Hu22 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu28 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu29 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu24 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu25 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu26 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB230Hu27 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB230Hu | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB230Hu | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PGB230Hu02 | 细胞毒性T-淋巴细胞关联抗原4(CTLA4)引物对 | PCR |
参考文献
| 杂志 | 参考文献 |
| Int Immunopharmacol | Circulating CTLA-4 levels and CTLA4 polymorphisms associate with disease condition and progression and hepatocellular carcinoma patients' survival in chronic … [Pubmed: 32163858] |
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